Imaging & Pathology Facility Manager: Dr David Dinsdale
Summary of Research Interests
The Imaging & Pathology group is currently responsible for the characterisation of morphological/functional changes in cells and tissues provided by other groups within the Unit. These studies involve histological and ultrastructural procedures for tissue samples together with various techniques for examining both living and processed cells after isolation.
High content screening and high content analysis enables rapid evaluation of changes in the size, shape, labelling- intensity and other properties of adherent cells whereas the combination of flow-cytometric analysis with the physical sorting of isolated cells has been found to be particularly valuable to groups needing to compare defined cell populations.
More detailed information on individual cells and tissue samples is provided by light-, fluorescent- and confocal-microscopy. Some of these fluorescent molecules used in confocal-microscopy, together with constitutive proteins have been localised using immunogold cytochemistry at the ultrastructural level. We also have considerable experience in autoradiography, cytochemistry, energy-dispersive X-ray microanalysis, microinjection, negative staining and scanning electron microscopy.
Image analysis has proved to be valuable in speeding-up simple procedures, such as cell counting, but also the more complex tasks of stereology, 3-D rendering, and deconvolution.
All copyrights in this website, including logos, illustrations, photos, sound, scripts, animation and other material appearing on this website, otherwise known as "content", belong to the MRC Toxicology Unit or third parties authorising the MRC to use the copyrights.
Users must not reprint or electronically reproduce from the MRC Toxicology Unit website any graphic in whole or in part without the prior written permission of the MRC Toxicology Unit.
Users may download and save electronic documents, audio files and forms from the Website and print or listen to these for personal use, but such forms, files and documents remain the copyright of the MRC Toxicology Unit, 2013.
MRC Toxicology Unit
PO Box 138
Lancaster Road, Leicester
LE1 9HN, UK
SatNav Users: Using this postcode will not direct you to the correct location. Please search for Lancaster Road.
Tel: +44 (0)116 252 5544
Fax: +44 (0)116 252 5616